pAIO
Plasmid DNA from Escherichia coli for antibiotic-free genome integration.
The plasmid DNA was purified from Escherichia coli NEB5α and consists of 8280 bp (MW ~ 5.1 x106 Da). The pAIO plasmid is equipped with all elements for “All-In-One” genome integration. The λ-red enzymes Exo, Gam and Bet under control of a heat inducible promotor were selected to ensure the integration of a linear target gene cassette into the E. coli genome. The gene encoding the homing endonuclease I-SceI was inserted into the vector under control of the araB promoter for selection purpose and plasmid curing. To provide accelerated plasmid curing, we engineered an additional I-SceI site into the plasmid backbone, with the idea that the plasmid also gets cleaved upon the expression of I-SceI.
Material for Research and Development purposes only.
Please remember to acknowledge the contributors of this material by citing or mentioning them appropriately in any related publications.
| Storage temperature | 20 °C |
| Delivery condition | The plasmid is supplied in a solution of 5 mM Tris/HCl pH8.5 |
Publication
Egger E, Tauer C, Cserjan-Puschmann M, Grabherr R, Striedner G. Fast and antibiotic free genome integration into Escherichia coli chromosome. Sci Rep. 2020 Oct 5;10(1):16510. doi: 10.1038/s41598-020-73348-x. PMID: 33020519; PMCID: PMC7536200.
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