pAIO
Plasmid DNA from Escherichia coli for antibiotic-free genome integration.
The plasmid DNA was purified from Escherichia coli NEB5α and consists of 8280 bp (MW ~ 5.1 x106 Da). The pAIO plasmid is equipped with all elements for “All-In-One” genome integration. The λ-red enzymes Exo, Gam and Bet under control of a heat inducible promotor were selected to ensure the integration of a linear target gene cassette into the E. coli genome. The gene encoding the homing endonuclease I-SceI was inserted into the vector under control of the araB promoter for selection purpose and plasmid curing. To provide accelerated plasmid curing, we engineered an additional I-SceI site into the plasmid backbone, with the idea that the plasmid also gets cleaved upon the expression of I-SceI.
Material for Research and Development purposes only.
Please remember to acknowledge the contributors of this material by citing or mentioning them appropriately in any related publications.
| Storage condition | -20 °C |
| Delivery condition | The plasmid is supplied in a solution of 5 mM Tris/HCl pH8.5. |
Publication
Fink M, Vazulka S, Egger E, Jarmer J, Grabherr R, Cserjan-Puschmann M, Striedner G. Microbioreactor Cultivations of Fab-Producing Escherichia coli Reveal Genome-Integrated Systems as Suitable for Prospective Studies on Direct Fab Expression Effects. Biotechnol J. 2019 Nov;14(11):e1800637. doi: 10.1002/biot.201800637. Epub 2019 Jul 26. PMID: 31231932.
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