BL21(DE3)::I-SceIRS
Escherichia coli host strain carrying the I-SceI restriction site on genome enabling selection during antibiotic free genome integration on attTN7 site.
BL21(DE3)::I-SceIRS (pAIO) offers a I-SceI homing endonuclease restriction site on genome. The 18 nucleotides long sequence was inserted at the attTN7 site and allows for selection during pAIO-based genome integration. (doi: 10.1038/s41598-020-73348-x).
Material for Research and Development purposes only.
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| Storage temperature | 4 °C |
| Strain | Escherichia coli |
| Delivery condition | The strain is provided on a lysogeny broth (LB) agar plate. |
Publication
Fink M, Vazulka S, Egger E, Jarmer J, Grabherr R, Cserjan-Puschmann M, Striedner G. Microbioreactor Cultivations of Fab-Producing Escherichia coli Reveal Genome-Integrated Systems as Suitable for Prospective Studies on Direct Fab Expression Effects. Biotechnol J. 2019 Nov;14(11):e1800637. doi: 10.1002/biot.201800637. Epub 2019 Jul 26. PMID: 31231932.
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