BL21(DE3)::I-SceIRS
Escherichia coli host strain carrying the I-SceI restriction site on genome enabling selection during antibiotic free genome integration on attTN7 site.
BL21(DE3)::I-SceIRS (pAIO) offers a I-SceI homing endonuclease restriction site on genome. The 18 nucleotides long sequence was inserted at the attTN7 site and allows for selection during pAIO-based genome integration. (doi: 10.1038/s41598-020-73348-x).
Material for Research and Development purposes only.
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| Delivery condition | The strain is provided on a lysogeny broth (LB) agar plate. |
| Strain | Escherichia coli |
| Storage temperature | 4 °C |
Publication
Egger E, Tauer C, Cserjan-Puschmann M, Grabherr R, Striedner G. Fast and antibiotic free genome integration into Escherichia coli chromosome. Sci Rep. 2020 Oct 5;10(1):16510. doi: 10.1038/s41598-020-73348-x. PMID: 33020519; PMCID: PMC7536200.
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