H<oFTN2>
Escherichia coli host strain for genome integrated Fab FTN2 expression strain.
H<oFTN2> originates from an Escherichia coli HMS174(DE3) strain and contains a Fab FTN2 expression cassette on the attTn7 site of the genome. FTN2 is a humanized Fab that neutralizes tumor necrosis factor α. For FTN2 translocation into the periplasmic space, the light chain (LC) and the heavy chain (HC) of the Fab were fused to the outer membrane protein A signal sequence (ompA_SS), which utilizes the post-translational SecB-pathway.
The expression cassette is composed of the T7 promoter/operator element (highlighted in yellow), the coding sequence for the ompA signal sequence for periplasmic translocation (highlighted in green), the Fab light chain (LC) (highlighted in magenta), a spacer and again the ompA signal sequence, the FTN2 heavy chain (HC) (highlighted in red) and the transcription terminator element tZenit (highlighted in blue).
| Delivery condition | Prepare stocks from single colony upon receipt. Store plate at 4°C. |
| Strain | Escherichia coli |
| Storage temperature | 4 °C |
Publication
Fink M, Vazulka S, Egger E, Jarmer J, Grabherr R, Cserjan-Puschmann M, Striedner G. Microbioreactor Cultivations of Fab-Producing Escherichia coli Reveal Genome-Integrated Systems as Suitable for Prospective Studies on Direct Fab Expression Effects. Biotechnol J. 2019 Nov;14(11):e1800637. doi: 10.1002/biot.201800637. Epub 2019 Jul 26. PMID: 31231932.
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