pET30acer_oFTN2
Plasmid DNA from Escherichia coli for T7RNAP-based FTN2 expression
The pET30acer_oFTN2 vector enables T7 RNA polymerase-based expression of FTN2, which is a humanized Fab that neutralizes tumor necrosis factor α. For FTN2 translocation into the periplasmic space, the light chain (LC) and the heavy chain (HC) of the Fab were fused to the outer membrane protein A signal sequence (ompA_SS), which utilizes the post-translational SecB-pathway. doi: 10.1002/BIOT.201800637.
Transcription termination is initialized by the strong terminator tZenit, consisting of 3 single termination loops. More precisely, tZenit includes a synthetically improved T7 terminator (T7T UUCG), a rrnBT1 termination sequence, and a native T7 terminator. doi:10.1021/sb5000115.
The cer element provides additional stability by preventing the formation of plasmid di- and multimers via recombination events catalysed by host cell enzymes. doi: 10.1002/j.1460-2075.1988.tb02884.x.
The plasmid DNA was purified from Escherichia coli NEB5α and consists of 6783 bp (MW ~ 4.2 x106 Da).
Material for Research and Development purposes only.
Please remember to acknowledge the contributors of this material by citing or mentioning them appropriately in any related publications.
| Storage temperature | -20 °C |
| Delivery condition | The plasmid is supplied in a solution of 5 mM Tris/HCl pH8.5. |
Publication
Fink M, Vazulka S, Egger E, Jarmer J, Grabherr R, Cserjan-Puschmann M, Striedner G. Microbioreactor Cultivations of Fab-Producing Escherichia coli Reveal Genome-Integrated Systems as Suitable for Prospective Studies on Direct Fab Expression Effects. Biotechnol J. 2019 Nov;14(11):e1800637. doi: 10.1002/biot.201800637. Epub 2019 Jul 26. PMID: 31231932.
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